Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.

نویسندگان

  • Aneta J Gubala
  • David F Proll
چکیده

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 72 9  شماره 

صفحات  -

تاریخ انتشار 2006